Category Archives: Molecular Organism

Inhibition of the deubiquitinase USP8 corrects a Drosophila PINK1 model of mitochondria dysfunction

Von Stockum S, Sanchez-Martinez A, Corrà S, Chakraborty J, Marchesan E, Locatello L, Da-Rè C, Cusumano P, Caicci F, Ferrari V, Costa R, Bubacco L, Rasotto MB, Szabo I, Whitworth AJ, Scorrano L, Ziviani E Life Science Alliance Aberrant mitochondrial dynamics disrupts mitochondrial function and contributes to disease conditions. A targeted RNA interference screen for […]

Knockdown of APOPT1/COA8 Causes Cytochrome c Oxidase Deficiency, Neuromuscular Impairment, and Reduced Resistance to Oxidative Stress in Drosophila melanogaster

Brischigliaro M, Corrà S, Tregnago C, Fernandez-Vizarra E, Zeviani M, Costa R, De Pittà C Frontiers in Physiology Cytochrome c oxidase (COX) deficiency is the biochemical hallmark of several mitochondrial disorders, including subjects affected by mutations in apoptogenic-1 (APOPT1), recently renamed as COA8 (HGNC:20492). Loss-of-function mutations are responsible for a specific infantile or childhood-onset mitochondrial leukoencephalopathy with a chronic clinical course. […]

Peptidergic signaling from clock neurons regulates reproductive dormancy in Drosophila melanogaster.

Nagy D, Cusumano P, Andreatta G, Anduaga AM, Hermann-Luibl C, Reinhard N, Gesto J, Wegener C, Mazzotta G, Rosato E, Kyriacou CP, Helfrich-Förster C, Costa R. PLoS Genetics With the approach of winter, many insects switch to an alternative protective developmental program called diapause. Drosophila melanogaster females overwinter as adults by inducing a reproductive arrest that is […]

The RNA helicase BELLE is involved in circadian rhythmicity and in transposons regulation in Drosophila melanogaster

Paola Cusumano, Milena Damulewicz, Elena Carbognin, Laura Caccin, Antonietta Puricella, Valeria Specchia, Maria Pia Bozzetti, Rodolfo Costa, Gabriella M. Mazzotta Frontiers in Physiology Circadian clocks control and synchronize biological rhythms of several behavioral and physiological phenomena in most, if not all, organisms. Rhythm generation relies on molecular auto-regulatory oscillations of interlocked transcriptional-translational feedback loops. Rhythmic clock-gene […]

Cryptochrome Is a Regulator of Synaptic Plasticity in the Visual System of Drosophila melanogaster

Damulewicz M, Mazzotta GM, Sartori E, Rosato E, Costa R, Pyza EM Frontiers in Molecular Neuroscience, 2017 Drosophila CRYPTOCHROME (CRY) is a blue light sensitive protein with a key role in circadian photoreception. A main feature of CRY is that light promotes an interaction with the circadian protein TIMELESS (TIM) resulting in their ubiquitination and degradation, a […]

Calmodulin Enhances Cryptochrome Binding to INAD in Drosophila Photoreceptors.

Mazzotta GM, Bellanda M, Minervini G, Damulewicz M, Cusumano P, Aufiero S, Stefani M, Zambelli B, Mammi S, Costa R, Tosatto SCE. Frontiers in Molecular Neuroscience, 2018 Light is the main environmental stimulus that synchronizes the endogenous timekeeping systems in most terrestrial organisms. Drosophila cryptochrome (dCRY) is a light-responsive flavoprotein that detects changes in light […]

Modulation of miR-210 alters phasing of circadian locomotor activity and impairs projections of PDF clock neurons in Drosophila melanogaster.

Cusumano P, Biscontin A, Sandrelli F, Mazzotta GM, Tregnago C, De Pittà C, Costa R. Plos Genetics Single microRNAs are usually associated with hundreds of putative target genes that can influence multiple phenotypic traits in Drosophila, ranging from development to behaviour. We investigated the function of Drosophila miR-210 in circadian behaviour by misexpressing it within […]

Cryptochrome Interacts With Actin and Enhances Eye-Mediated Light Sensitivity of the Circadian Clock in Drosophila melanogaster.

Schlichting M, Rieger D, Cusumano P, Grebler R, Costa R, Mazzotta GM, Helfrich-Förster C. Frontiers in Molecular Neuroscience Cryptochromes (CRYs) are a class of flavoproteins that sense blue light. In animals, CRYs are expressed in the eyes and in the clock neurons that control sleep/wake cycles and are implied in the generation and/or entrainment of […]

A Semi-natural Approach for Studying Seasonal Diapause in Drosophila melanogaster Reveals Robust Photoperiodicity

Nagy D.,  Andreatta G., Bastianello S., Martín Anduaga A., Mazzotta G., Kyriacou CP., Costa R. Journal of Biological Rhythms The fruit fly Drosophila melanogaster survives thermally stressful conditions in a state of reproductive dormancy (diapause), manifested by reduced metabolic activity and arrested ovarian development in females. Unlike insects that rely primarily on photoperiodic stimuli to initiate […]